diff --git a/django/db/models/sql/query.py b/django/db/models/sql/query.py
index 9fde8496e9..4d31c36175 100644
--- a/django/db/models/sql/query.py
+++ b/django/db/models/sql/query.py
@@ -1221,7 +1221,7 @@ class Query(BaseExpression):
         else:
             self.set_annotation_mask(set(self.annotation_select).difference({alias}))
         self.annotations[alias] = annotation
-        if self.selected:
+        if select and self.selected:
             self.selected[alias] = alias
 
     @property
diff --git a/docs/releases/5.2.1.txt b/docs/releases/5.2.1.txt
index 0f95eda848..53f18152ae 100644
--- a/docs/releases/5.2.1.txt
+++ b/docs/releases/5.2.1.txt
@@ -19,3 +19,7 @@ Bugfixes
 * Fixed a regression in Django 5.2 that caused a crash of
   ``QuerySet.bulk_create()`` with nullable geometry fields on PostGIS
   (:ticket:`36289`).
+
+* Fixed a regression in Django 5.2 that caused fields to be incorrectly
+  selected when using ``QuerySet.alias()`` after ``values()``
+  (:ticket:`36299`).
diff --git a/tests/annotations/tests.py b/tests/annotations/tests.py
index 5df958c333..6c0d7b668c 100644
--- a/tests/annotations/tests.py
+++ b/tests/annotations/tests.py
@@ -1470,6 +1470,10 @@ class AliasTests(TestCase):
                 with self.assertRaisesMessage(FieldError, msg):
                     getattr(qs, operation)("rating_alias")
 
+    def test_alias_after_values(self):
+        qs = Book.objects.values_list("pk").alias(other_pk=F("pk"))
+        self.assertEqual(qs.get(pk=self.b1.pk), (self.b1.pk,))
+
     def test_alias_sql_injection(self):
         crafted_alias = """injected_name" from "annotations_book"; --"""
         msg = (