diff --git a/django/db/models/fields/tuple_lookups.py b/django/db/models/fields/tuple_lookups.py
index 161e2973a0..6c21263b6a 100644
--- a/django/db/models/fields/tuple_lookups.py
+++ b/django/db/models/fields/tuple_lookups.py
@@ -2,7 +2,13 @@ import itertools
 
 from django.core.exceptions import EmptyResultSet
 from django.db.models import Field
-from django.db.models.expressions import ColPairs, Func, ResolvedOuterRef, Value
+from django.db.models.expressions import (
+    ColPairs,
+    Func,
+    ResolvedOuterRef,
+    Subquery,
+    Value,
+)
 from django.db.models.lookups import (
     Exact,
     GreaterThan,
@@ -290,7 +296,7 @@ class TupleIn(TupleLookupMixin, In):
             )
 
     def check_rhs_is_query(self):
-        if not isinstance(self.rhs, Query):
+        if not isinstance(self.rhs, (Query, Subquery)):
             lhs_str = self.get_lhs_str()
             rhs_cls = self.rhs.__class__.__name__
             raise ValueError(
diff --git a/tests/composite_pk/test_filter.py b/tests/composite_pk/test_filter.py
index d4c6ef13e0..c633348cc1 100644
--- a/tests/composite_pk/test_filter.py
+++ b/tests/composite_pk/test_filter.py
@@ -442,6 +442,11 @@ class CompositePKFilterTests(TestCase):
         with self.assertRaisesMessage(ValueError, msg):
             Comment.objects.filter(text__gt=Cast(F("pk"), TextField())).count()
 
+    def test_explicit_subquery(self):
+        subquery = Subquery(User.objects.values("pk"))
+        self.assertEqual(User.objects.filter(pk__in=subquery).count(), 4)
+        self.assertEqual(Comment.objects.filter(user__in=subquery).count(), 5)
+
     def test_filter_case_when(self):
         msg = "When expression does not support composite primary keys."
         with self.assertRaisesMessage(ValueError, msg):